A high throughput Brassica napus microspore culture system: influence of percoll gradient separation and bud selection on embryogenesis
نویسندگان
چکیده
منابع مشابه
Microspore culture reveals high fitness of B. napus-like gametes in an interspecific hybrid between Brassica napus and B. oleracea
The strategies of crossing B. napus with parental species play important role in broadening and improving the genetic basis of B. napus by the introgression of genetic resources from parental species. With these strategies, it is easy to select new types of B. napus, but difficult to select new types of B. rapa or B. oleracea by self-pollination. This characteristic may be a consequence of high...
متن کاملDynamics of Calcium during In vitro Microspore Embryogenesis and In vivo Microspore Development in Brassica napus and Solanum melongena
Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. ...
متن کاملTranscript profiling and identification of molecular markers for early microspore embryogenesis in Brassica napus.
Isolated microspores of Brassica napus are developmentally programmed to form gametes; however, microspores can be reprogrammed through stress treatments to undergo appropriate divisions and form embryos. We are interested in the identification and isolation of factors and genes associated with the induction and establishment of embryogenesis in isolated microspores. Standard and normalized cDN...
متن کاملIsolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis
Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the ...
متن کاملEffects of gibberellin, abscisic acid and embryo desiccation on normal plantlet regeneration, secondary embryogenesis and callogenesis in microspore culture of Brassica napus L. cv. PF704
Most of the microspore-derived embryos can not regenerate normally in rapeseed. The effects of gibberellins (GA3), abscisic acid (ABA), and embryo desiccation on normal plantlet regeneration were studied. The donor plants were grown in a growth chamber at day/night temperatures of 15/10˚C with a 16/8h photoperiod, respectively. Microspores were isolated from whole buds of 2.5-3.5mm in length, c...
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture (PCTOC)
سال: 2011
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-010-9913-3